Affymetrix

Overview

Affymetrix GeneChip arrays are high-density oligonucleotide microarrays manufactured using a photolithographic technology. This system allows you to measure the relative concentration of an RNA or DNA sequence in a complex mixture of nucleic acids. Expression arrays enable researchers to simultaneously monitor genome-wide expression profiles, while Mapping Arrays enable large-scale linkage analysis, association, and copy number studies.

Equipment:

The Array Facility at Charterhouse Square, was established with funding from The Special Trustees and consists of a complete Affymetrix GeneChip System comprising two Fluidics 450 Wash Stations, two Hybridisation Ovens and a GeneChip Scanner 3000 7G. The equipment we have allows for efficient and concurrent throughput of both Gene Expression and SNP Genotyping chips.

Services:

We provide a chip hybridization service for The Barts Cancer Institute at Charterhouse Square and we are available to the College and External researchers. Advice is offered on experimental design, probe making and chip ordering.

If you wish to discuss a more comprehensive service of probe making, please contact the Services Manager for discussion and costs.

Now including:

• NuGen WT Ovation kit for extraction of RNA from FFPE samples and OncoScan™ FFPE Express enabling integrated analysis of whole-genome copy number changes, copy-neutral loss of heterozygosity (LOH) and somatic mutations in archived FFPE samples.
• Expression analysis at both Gene and Exon level in Human, Mouse and Rat, including the recently launched GeneChip® miRNA 2.0 Array, for use as a complement to (mRNA) gene expression studies

Booking and Charges:

A ‘flexible’ booking system is operated and you are expected to provide your own chips and sample cocktail. Experimental data is provided on CD for your analysis.

You are encouraged to track the quality of your probe using our Agilent 2100 Bioanalyser which utilizes a "Lab-on-a-Chip” system to assess total RNA and fragmented cRNA quality.

Charges can be found here

Please contact the service manager to book.